2BS1
MS2 (N87AE89K mutant) - Qbeta RNA hairpin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 273.100, 273.500, 273.400 |
| Unit cell angles | 63.26, 63.11, 63.31 |
Refinement procedure
| Resolution | 29.940 - 2.800 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 347537 | |
| <I/σ(I)> | 15 | 10 |
| Completeness [%] | 23.0 | 10 |
| Redundancy | 1.3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
