2BS0
MS2 (N87AE89K mutant) - Variant Qbeta RNA hairpin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 274.200, 273.000, 273.900 |
| Unit cell angles | 63.06, 62.98, 63.28 |
Refinement procedure
| Resolution | 29.920 - 2.450 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.040 | 0.300 |
| Number of reflections | 460526 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 20.0 | 6 |
| Redundancy | 1.3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
