2BRV
Crystal structure of Streptococcus Pneumoniae Hyaluronate Lyase from 70percent saturated malonate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD INSTRUMENTS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.722, 101.052, 85.211 |
| Unit cell angles | 90.00, 125.12, 90.00 |
Refinement procedure
| Resolution | 69.010 - 3.300 |
| R-factor | 0.302 |
| Rwork | 0.301 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1egu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.170 | 0.610 |
| Number of reflections | 11046 | |
| <I/σ(I)> | 6.3 | 2.3 |
| Completeness [%] | 93.8 | 84 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | pH 7.60 |
