2BR6
Crystal Structure of Quorum-Quenching N-Acyl Homoserine Lactone Lactonase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-20 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.904, 55.745, 73.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.700 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.640 |
| Number of reflections | 23923 | |
| <I/σ(I)> | 19.4 | 3.1 |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 0.1 M TRIS-HCL(PH 7.8), 30% PEG 4000,0.2 M MGCL2, 2 MM HOMOSERINE LACTONE |
