2BQY

Inorganic Pyrophosphatase from the Pathogenic Bacterium Helicobacter pylori-Kinetic and Structural Properties

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-15
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	101.500, 101.500, 93.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.910 - 2.300
R-factor	0.25
Rwork	0.250
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.480
Number of reflections	146975
<I/σ(I)>	24	4.7
Completeness [%]	99.3	100
Redundancy	11	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1