2BQ0
14-3-3 Protein Beta (Human)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-08 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.605, 123.790, 54.755 |
| Unit cell angles | 90.00, 114.98, 90.00 |
Refinement procedure
| Resolution | 61.900 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qja |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.227 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 21068 | |
| <I/σ(I)> | 5.1 | 2 |
| Completeness [%] | 97.7 | 96.6 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.05M MGCL2,0.1M HEPES PH7.5, 30% PEG MME 550, pH 8.00 |
