2BPX

HIV-1 protease-inhibitor complex

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	58.600, 87.370, 46.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.800
R-factor	0.175
Rwork	0.175
RMSD bond length	0.011
RMSD bond angle	1.980
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.070 *
Total number of observations	25963 *
Number of reflections	6385 *
Completeness [%]	87.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6 (mg/ml)
2	1	drop	MES	10 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop		3 (mM)
6	1	reservoir		0.6 (M)
7	1	reservoir		0.1 (M)