2BPS

Ubiquitin-like protein YukD of Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	50.328, 50.328, 175.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	200.000 - 2.700
R-factor	0.241
Rwork	0.241
R-free	0.29630
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.840
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.410
Number of reflections	6767
<I/σ(I)>	12.2	2.7
Completeness [%]	99.3	99.3
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1