2BPD
STRUCTURE OF MURINE DECTIN-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 57.104, 57.104, 73.422 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1K9J 1fm5 1hq8 1ja3 1mpu 1e87 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.336 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.570 |
| Number of reflections | 41593 | |
| <I/σ(I)> | 28.1 | 1.7 |
| Completeness [%] | 97.0 | 78.1 |
| Redundancy | 4.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M DI-AMMONIUM HYDROGEN CITRATE, 20% PEG 3350 |
