2BPB
Sulfite dehydrogenase from Starkeya Novella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.611, 93.167, 56.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.21000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.451 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.440 |
| Number of reflections | 40189 | |
| <I/σ(I)> | 12.5 | 2.8 |
| Completeness [%] | 97.9 | 97.7 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | .1M HEPES PH 7.4, 2.2M AMMONIUM SULFATE, 2% PEG200 |
