2BPA

ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 1 21 1
Unit cell lengths	305.580, 360.780, 299.460
Unit cell angles	90.00, 92.89, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.209
RMSD bond length	0.016
RMSD bond angle	3.100
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.129 *
Number of reflections	1000304 *	92108 *
Completeness [%]		27 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			referred to J.Mol.Biol. 212.345-350 1990 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bis-Tris methane	90-93 (mM)
2	1	reservoir	PEG8000	1.5-2.0 (%(w/v))
3	1	drop	virus	0.125 (mg/ml)
4	1	drop	bis-Tris methane	90-93 (mM)
5	1	drop	PEG8000	1.5-2.0 (%(w/v))