2BOU
EGF Domains 1,2,5 of human EMR2, a 7-TM immune system molecule, in complex with barium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.060, 61.560, 35.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.299 - 1.900 |
| R-factor | 0.2384 |
| Rwork | 0.238 |
| R-free | 0.25470 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.260 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 14758 | |
| <I/σ(I)> | 4.9 | 1.1 |
| Completeness [%] | 90.3 | 57.9 |
| Redundancy | 22.2 | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1-0.175 M BARIUM CHLORIDE, 12-16% W/V PEG 8000, 0.1 M NA CACODYLATE BUFFER PH 6.5 |
