2BOJ
crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with methyl-B-D-Arabinopyranoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-10 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.636, 80.168, 52.517 |
Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.800 |
R-factor | 0.137 |
Rwork | 0.136 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.190 |
Number of reflections | 35769 | |
<I/σ(I)> | 7.5 | 3.64 |
Completeness [%] | 97.9 | 89 |
Redundancy | 3.73 | 3.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMONIUM SULFATE, pH 8.50 |