2BOJ
crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with methyl-B-D-Arabinopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.636, 80.168, 52.517 |
| Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.800 |
| R-factor | 0.137 |
| Rwork | 0.136 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 35769 | |
| <I/σ(I)> | 7.5 | 3.64 |
| Completeness [%] | 97.9 | 89 |
| Redundancy | 3.73 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMONIUM SULFATE, pH 8.50 |
