2BOB
Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-08-08 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 155.267, 155.267, 75.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.760 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.930 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 21783 | |
<I/σ(I)> | 6.9 | 2.11 |
Completeness [%] | 95.7 | 95.7 |
Redundancy | 3.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | PEG 400, MAGNESIUM ACETATE, SODIUM ACETATE, pH 5.40 |