2BOA
Human procarboxypeptidase A4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.800, 87.200, 89.700 |
| Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aye |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.330 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 46526 | |
| <I/σ(I)> | 10.5 | 2.2 |
| Completeness [%] | 97.9 | 95.9 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 14.5% PEG4000; 0.1M BICINE PH 9.0; 0.2M MAGCL2. |
