2BNY
MS2 (N87A mutant) - RNA hairpin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 288.000, 288.000, 653.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.960 - 3.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.230 |
| Number of reflections | 102199 | |
| Completeness [%] | 49.0 | 14 |
| Redundancy | 1.5 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
