2BNO
The structure of Hydroxypropylphosphonic acid epoxidase from S. wedmorenis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.430, 86.430, 221.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 39606 | |
| <I/σ(I)> | 26.6 | 2.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.1 M AMMONIUM SULPHATE, 100 MM TRIS-HCL PH 7.5 |
