2BNN
The structure of Hydroxypropylphosphonic acid epoxidase from S. wedmorenis in complex with fosfomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 85.602, 85.602, 217.989 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.27000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.262 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.670 |
| Number of reflections | 81586 | |
| <I/σ(I)> | 12.3 | 2.3 |
| Completeness [%] | 97.9 | 96.5 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.1 M AMMONIUM SULPHATE, 100 MM TRIS PH 7.5 |
