2BNL
The structure of the N-terminal domain of RsbR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 136.062, 136.062, 113.296 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.240 - 2.000 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.112 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.250 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.340 |
| Number of reflections | 80643 | |
| <I/σ(I)> | 3.1 | 2.24 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 21.05 | 13.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | EQUAL VOLUMES OF 1.8M SODIUM MALONATE PH 8.0 AND 10MG/ML N-RSBR PROTEIN IN A HANGING DROP ABOVE A WELL OF 1.8M SODIUM MALONATE PH 8.0. |
