2BNJ
The xylanase TA from Thermoascus aurantiacus utilizes arabinose decorations of xylan as significant substrate specificity determinants.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-26 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.224, 49.462, 77.384 |
| Unit cell angles | 90.00, 125.38, 90.00 |
Refinement procedure
| Resolution | 63.120 - 1.600 |
| R-factor | 0.117 |
| Rwork | 0.115 |
| R-free | 0.14700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i1x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.450 | 1.610 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 0.070 |
| Number of reflections | 34409 | |
| <I/σ(I)> | 25.12 | 9.31 |
| Completeness [%] | 95.8 | 88 |
| Redundancy | 2.64 | 2.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 13.5% PEG 6K, 2.5MM FAX2, 4MG/ML TA PROTEIN. |
