2BMJ
GTPase like domain of Centaurin Gamma 1 (Human)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-09 |
| Detector | RIGAKU/MSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.151, 31.104, 81.928 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 80.060 - 2.100 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rap |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.528 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.220 |
| Number of reflections | 43169 | |
| <I/σ(I)> | 7.5 | 2.9 |
| Completeness [%] | 95.1 | 89.9 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M SPG PH 7.0; 30.0% PEG1000; 0.5% DMSO |
