Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.256, 94.920, 111.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | AMoRE ((NAVAZA)) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 * | |
| Total number of observations | 191117 * | |
| Number of reflections | 32806 | |
| Completeness [%] | 94.9 * | 91.8 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 288 * | Carfi, A., (1997) Acta Crystallogr., D53, 485. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.0 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 22-24 (%(w/v)) | |
| 3 | 1 | reservoir | dithiothreitol | 0.100 (mM) | |
| 4 | 1 | reservoir | Bicine | 100 (mM) | |
| 5 | 1 | reservoir | 0.2-1 (mM) | ||
| 6 | 1 | reservoir | glycerol | 2-5 (%) |
