2BM5
The Structure of MfpA (Rv3361c, P21 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | MSC RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.821, 31.018, 96.846 |
| Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PREVIOUS STRUCTURE DETERMINED BY SE-MET MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.894 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.030 | 0.160 |
| Number of reflections | 21905 | |
| <I/σ(I)> | 29.6 | 7.4 |
| Completeness [%] | 98.8 | 95.8 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | PROTEIN (10 MG/ML, 30 MM BETA-MERCAPTOETHANOL, 10 MM AMMONIUM CITRATE) CRYSTALLIZED IN 30% ETHYLENE GLYCOL, 100 MM CITRATE PHOSPHATE PH5.5, 200 MM AMMONIUM SULFATE., pH 5.50 |
