2BM1
Ribosomal elongation factor G (EF-G) Fusidic acid resistant mutant G16V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.300, 89.700, 114.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.600 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 24149 | |
| <I/σ(I)> | 14 | 3.2 |
| Completeness [%] | 98.2 | 97.2 |
| Redundancy | 8.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 17 % PEG8000 100 MM HEPES 46 MM TRIS-HCL PH 7.3 10 MM MAGNESIUM CHLORIDE |
