2BLS
AMPC BETA-LACTAMASE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.730, 77.350, 97.590 |
| Unit cell angles | 90.00, 116.45, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BLT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 19.200 * |
| Data reduction software | MSC |
| Data scaling software | MSC |
| Phasing software | AMoRE |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.183 |
| Total number of observations | 73349 * | |
| Number of reflections | 41301 | 9094 * |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 77.4 | 58.1 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.7 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 1.7 (M) | |
| 2 | 1 | drop | protein | 10 (mg/ml) |
