2BLP
RNase before unattenuated X-RAY burn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.132, 64.132, 63.643 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 1.400 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.17600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.040 | 0.240 |
| Number of reflections | 55873 | |
| <I/σ(I)> | 10.93 | 3.25 |
| Completeness [%] | 96.8 | 90.3 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 30 % PEG 5000 MME,1 M SODIUM CHLORIDE,50 MM PH 4.5 SODIUM ACETATE |
