2BLN
N-terminal formyltransferase domain of ArnA in complex with N-5- formyltetrahydrofolate and UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.176, 89.957, 97.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.700 - 1.200 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.15800 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.870 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.900 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 183672 | |
| <I/σ(I)> | 7 | 2.4 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M AMMONIUM ACETATE, 0.1 M BIS-TRIS PH 5.5, 17 % PEG 10000 |
