2BL7

1.6 Angstrom crystal structure of EntA-im: a bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin, enterocin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	75.800, 75.800, 36.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.2097
Rwork	0.210
R-free	0.26060
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.008
Data reduction software	MOSFLM
Data scaling software	SCALEIT
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	0.400
Number of reflections	6364
<I/σ(I)>	16.5	2.2
Completeness [%]	99.8	98.8
Redundancy	8.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1