2BL0

Physarum polycephalum myosin II regulatory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	55.574, 70.858, 97.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.740 - 1.750
R-factor	0.221
Rwork	0.219
R-free	0.25200
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.223
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.700	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.040	0.370
Number of reflections	39847
<I/σ(I)>	13	3
Completeness [%]	99.4	99
Redundancy	6.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN SOL: 8.5 MG/ML PHYS RD IN 10 MM TRIS PH 7.6, 10 MM CACL2, RESERVOIR SOL: 20% PEG4000, 0.5 M CAAC PH 4.6, 0.1 M NH4AC, 0.05 M NACL