2BKO

STRUCTURE ANALYSIS OF UNKNOWN FUNCTION PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-30
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 42 21 2
Unit cell lengths	70.860, 70.860, 98.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vct
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.380
Number of reflections	20450
<I/σ(I)>	25	3.9
Completeness [%]	99.9	99.8
Redundancy	5.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.7		PROTEIN WAS CRYSTALLIZED FROM 1.25 M NACL, 50 MM ACETATE, PH 4.7; THEN SOAKED WITH 1.5 M CACL2.