2BJV
Crystal Structure of PspF(1-275) R168A mutant
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-28 |
| Detector | ADSC |
| Wavelength(s) | 0.97, 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.613, 113.613, 39.145 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.000 - 1.700 |
| R-factor | 0.17235 |
| Rwork | 0.172 |
| R-free | 0.21244 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.277 |
| Number of reflections | 29411 | |
| <I/σ(I)> | 26 | 3 |
| Completeness [%] | 91.6 | 60.5 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2 M AMMONIUM FORMATE, 0.1 MM HEPES PH 8.0, 10% MPD |
