2BJR
Crystal structure of the nematode sperm cell motility protein MFP2B
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.513, 52.954, 95.542 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.730 |
| Number of reflections | 74855 | |
| <I/σ(I)> | 21 | 3.1 |
| Completeness [%] | 93.0 | 96.9 |
| Redundancy | 14.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | PROTEIN WAS CRYSTALLIZED FROM 100 MM NA MES PH 4.5, 1.85 M AMMONIUM SULPHATE, 10 MM DTT, 10 MM ERCL3, 40 MM KSCN |
