2BJJ
Structure of recombinant human lactoferrin produced in the milk of transgenic cows
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.941, 97.379, 156.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 2.400 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lfg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 33492 | |
| <I/σ(I)> | 8.9 | 2.2 |
| Completeness [%] | 96.2 | 97.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 5 MM SODIUM PHOSPHATE PH 8.5, 10% (V/V) ETHANOL |
