2BJG
Crystal Structure of Conjugated Bile Acid Hydrolase from Clostridium perfringens in Complex with Reaction Products Taurine and Deoxycholate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 63.784, 63.784, 341.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 34729 | |
| <I/σ(I)> | 19 | 8.4 |
| Completeness [%] | 81.0 | 84 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG4000, 200 MM AMMONIUMSULFATE, 100 MM BISTRIS PH 5.5 |
