2BJ9
NIKR with bound NICKEL and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-15 |
| Detector | RIGAKU RAXIS-V |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.536, 108.536, 78.818 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.130 - 3.000 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.23200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 10906 | |
| <I/σ(I)> | 17.2 | 2.4 |
| Completeness [%] | 98.3 | 96.2 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
