2BJ6

Crystal Structure of a decameric HNA-RNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-25
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	113.910, 113.910, 55.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.600
R-factor	0.217
Rwork	0.217
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.245
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.060
Number of reflections	11676
<I/σ(I)>	28.7	3.2
Completeness [%]	99.4	95.5
Redundancy	6.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.9M LISO4, 10MM MG(AC)2,0.1M TRIS/HCL, PH 8.0