2BJ1
NIKR IN OPEN CONFORMATION AND NICKEL BOUND TO HIGH-AFFINITY SITES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-05 |
Detector | RIGAKU RAXIS-V |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 99.893, 99.893, 97.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.640 - 3.000 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q5y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | DM |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.060 | 0.290 |
Number of reflections | 10315 | |
<I/σ(I)> | 20.8 | 4.8 |
Completeness [%] | 99.0 | 98.8 |
Redundancy | 9.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |