Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.675, 85.675, 99.793 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.540 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.540 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.170 |
| Number of reflections | 2160 | |
| <I/σ(I)> | 10.6 | 4.3 |
| Completeness [%] | 98.1 | 88.6 |
| Redundancy | 16.6 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PH6.5, 50MM CACL2, 30% PEG550, pH 6.50 |
