2BI0
RV0216, A conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdogfold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-08-02 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.933,0.979,0.975 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 78.750, 78.750, 180.309 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 91.290 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.883 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | RSPS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.420 |
| Number of reflections | 26988 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1UL PROTEIN (3.6MG/ML) IN 20MM TRIS PH 7.5, 150MM NACL, 10% GLYCEROL AND 1UL RESERVOIR 100MM MES PH 6.5, 1.3M AMMONIUM SULFATE, 4% PEG400, 10MM BETA-MERCAPTOETHANOL. |
