2BHZ
Crystal structure of Deinococcus radiodurans maltooligosyltrehalose trehalohydrolase in complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.145, 66.565, 153.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.700 - 1.200 |
| R-factor | 0.125 |
| Rwork | 0.125 |
| R-free | 0.14200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.366 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 187653 | |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 99.2 | 95.2 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% (W/V) PEG 2000 MME, 20% (W/V) MALTOSE, 0.1 M TRIS-HCL PH 8.5 AND 0.1 M MGCL2 |
