2BHW
PEA LIGHT-HARVESTING COMPLEX II AT 2.5 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-02 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.400, 128.000, 62.000 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.2196 |
| Rwork | 0.220 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EM MAP |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.727 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 47867 | |
| <I/σ(I)> | 8.99 | 2.7 |
| Completeness [%] | 85.6 | 70.7 |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 14 % PEG 350 MMME 50 MM MES PH 5.5 10 % GLYCEROL, 20 MM NACL |
