2BHU
Crystal structure of Deinococcus radiodurans maltooligosyltrehalose trehalohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2003-07-30 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.580, 66.620, 152.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.410 - 1.100 |
| R-factor | 0.129 |
| Rwork | 0.128 |
| R-free | 0.15900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.797 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 216600 | |
| <I/σ(I)> | 7.1 | 2.3 |
| Completeness [%] | 88.3 | 82.9 |
| Redundancy | 2.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG 2000 MME, 0.1 M TRIS-HCL PH8.5, 0.1 M MGCL2, pH 8.50 |
