2BHN
XPF from Aeropyrum pernix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.325, 42.752, 118.736 |
| Unit cell angles | 90.00, 121.42, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.221 |
| Rwork | 0.216 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEPARATE DOMAINS FROM PART-REFINED PDB ENTRY 2BGW |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.703 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.930 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 52273 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 65% V/V MPD, 0.1M TRIS-HCL PH8.0. SITTING DROPS. |
