2BHA
E. coli Aminopeptidase P in complex with substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-26 |
Detector | MARRESEARCH |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 138.223, 138.223, 230.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.190 - 2.400 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1N51 STRIPPED OF MULTIPLE CONFORMERS SOLVENT ATOMS AND HETERO COMPOUNDS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.172 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.080 | 0.630 |
Number of reflections | 43763 | |
<I/σ(I)> | 20.2 | 2.4 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | AMINOPEPTIDASE P WAS DIALYSED AGAINST EGTA PRIOR TO CRYSTALLISATION. CRYSTALS WERE GROWN USING HANGING DROP VAOPR DIFFUSION AT 4C. RESERVOIR CONTAINED 26% MPD, 100 MM NA.CITRATE (PH 7.5) AND 200 MM MG.ACETATE. CRYSTALS WERE SOAKED IN RESERVOIR SOLUTION SUPPLEMENTED WITH 10 MM VALPROLEU TRIPEPTIDE FOR 1 HOUR AT 4C PRIOR TO DATA COLLECTION. |