2BGV
X-ray structure of ferric cytochrome c-550 from Paracoccus versutus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR78 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.102, 57.102, 66.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.440 - 1.900 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF M100K CYTOCHROME C-550 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.130 | 0.330 |
| Number of reflections | 9898 | |
| <I/σ(I)> | 20.5 | 1.24 |
| Completeness [%] | 99.9 | 97.7 |
| Redundancy | 14.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 3.2 M AMMONIUM SULFATE, 0.1 M BICINE PH 9 |
