2BGT

CRYSTAL STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE OF THE SUBSTRATE URIDINE DIPHOSPHOGLUCOSE

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	152.880, 52.250, 53.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.194
Rwork	0.194
RMSD bond length	0.011
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.084 *
Total number of observations	52070 *
Number of reflections	19074 *
Completeness [%]	84.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	ammonium sulfate	26 (%sat)
3	1	drop	UDPG	0.05 (mM)
4	1	reservoir	ammonium sulfate	52 (%sat)
5	1	reservoir	UDPG	0.1 (mM)
6	1	drop	sodium citrate	0.05 (M)
7	1	reservoir	sodium citrate	0.1 (M)