2BGJ
X-Ray Structure of the Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus at 2.1 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.290, 93.626, 103.427 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 105.410 - 2.100 |
| R-factor | 0.21555 |
| Rwork | 0.216 |
| R-free | 0.24322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.353 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 73447 | |
| <I/σ(I)> | 10.3 | 2.3 |
| Completeness [%] | 80.8 | 60.4 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
