2BEO
PrfA, Transcriptional Regulator In Listeria Monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.951, 80.713, 60.965 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 56.800 - 2.700 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.944 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.010 | 0.030 |
| Number of reflections | 12402 | |
| <I/σ(I)> | 10.6 | 3.1 |
| Completeness [%] | 89.0 | 68.9 |
| Redundancy | 1.76 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20 % (W/V) PEG 6000 100 MM NA-MES PH6.5 1 M LICL, pH 6.50 |
