2BEN
Crystal structure of the Serratia marcescens chitin-binding protein CBP21 Y54A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-09 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.447, 84.447, 82.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.780 - 1.800 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.470 |
| Number of reflections | 31629 | |
| <I/σ(I)> | 10.4 | 2.6 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG8000, 100 MM, CHAPS 200 MM NACL |
