2BEI

X-ray structure of thialysine n-acetyltransferase (SSAT2) from homo sapiens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97911, 0.96388
Spacegroup name	P 21 21 21
Unit cell lengths	54.583, 83.621, 87.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.774 - 1.842
R-factor	0.207
Rwork	0.204
R-free	0.24890
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.576
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.774	1.920
High resolution limit [Å]	1.842	1.842
Rmerge	0.077	0.459
Number of reflections	34249
<I/σ(I)>	10.833	2.377
Completeness [%]	97.0	78.5
Redundancy	6.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	10 MG/ML PROTEIN, 16.4% PEG 5K, 0.10 M MOPS, 0.15 M Potassium Glutamte, pH 7.0, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7	277	10 MG/ML PROTEIN, 16.4% PEG 5K, 0.10 M MOPS, 0.15 M Potassium Glutamte, pH 7.0, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP